Related Experiment Video
Updated: Aug 5, 2026

Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms
Published on: March 1, 2022
Synergistic Interactions Between Plant Growth-Promoting Bacteria and Trichoderma virens Enhance Arabidopsis Growth
Paulina Guzmán-Guzmán1, María D Ramírez-Ramos2, Ma Del Carmen Orozco-Mosqueda3
1Department of Chemical and Biochemical Engineering, Instituto Tecnológico de Morelia, Tecnológico Nacional de México, Morelia 58000, Mexico.
Abstract:
Trichoderma virens and plant growth-promoting bacteria (PGPB) are well-known agents that promote plant development and control pathogens. This study assessed the compatibility, biocontrol potential, and plant growth promotion of T. virens in combination with four PGPB strains (Pseudomonas fluorescens UM270, Rouxiella badensis SER3, Bacillus velezensis AF12, and Bacillus halotolerans AF23) against Fusarium brachygibbosum and Arabidopsis thaliana. The results showed that single inoculations significantly inhibited the growth of F. brachygibbosum by the 7th day of confrontation. However, co-inoculating T. virens with PGPB exhibited synergistic effects on the inhibition percentages for the consortia Tv + UM270 (48.94%), Tv + AF12 (67.04%), and Tv + SER3 (78.63%). Plant assays demonstrated that most microorganisms enhanced root development and plant height, with UM270 having the strongest beneficial effect. Expression analysis of T. virens effector genes (sm1, tvsep3, and tvhydii1) indicated early induction of tvhydii1 in the condition of Fb + AF12 at day 3, while sm1 was downregulated. No significant changes in the expression of these genes were detected during interaction with A. thaliana and PGPB. These findings demonstrate that T. virens-PGPB can simultaneously promote plant growth and suppress pathogens, with effector genes such as tvhydii1 contributing to these interactions, highlighting their potential for sustainable agriculture.
More Related Videos
11:16A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
Published on: July 22, 2017
09:04Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System
Published on: May 28, 2019
Related Concept Videos
Microbe-Plant Interactions
The Roles of Bacteria and Fungi in Plant Nutrition
Microbial Interactions: Cooperation
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Plant Breeding and Biotechnology
Microbial Interactions: Mutualism