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Proteomic Stability and Ex Vivo Compatibility of a Processed Phospholipoproteic Secretome-Derived Formulation
Ramón Gutiérrez-Sandoval1, Francisco Gutiérrez-Castro2, Natalia Muñoz-Godoy2
1OGRD Alliance LLC, 6900 Tavistock Lakes Boulevard, Suite 400, Lake Nona, Orlando, FL 32827, USA.
Pharmaceutics
|July 28, 2026
Summary
This study confirms that processed extracellular lipid-protein formulations maintain their protein composition and cellular compatibility after processing and storage. Proteomic analysis and cell viability assays show stability across various conditions, ensuring reliable analytical characterization.
Area of Science:
- Biochemistry and Molecular Biology
- Analytical Chemistry
- Cell Biology
Background:
- Processed extracellular lipid-protein preparations necessitate thorough analytical characterization.
- Ensuring compositional profile, processing stability, and short-term cellular compatibility is crucial across storage and handling.
Purpose of the Study:
- To quantitatively characterize a processed phospholipoproteic secretome-derived formulation under diverse conditions.
- To assess the preservation of proteomic profiles and cellular compatibility post-processing and storage.
Main Methods:
- Label-free quantitative proteomics using timsTOF Pro mass spectrometry with dia-PASEF.
- Comparative proteomic analysis across fresh, concentrated, cryopreserved, and lyophilized states.
- Real-time live-cell kinetic imaging on non-malignant dermal cells using the IncuCyte® S3 platform.
Main Results:
- Identification of 574 human proteins with >90% retention of structural proteins.
- High quantitative reproducibility (CVs 3.0-4.5%) and limited proteomic divergence.
- Stable cellular confluence kinetics and >92% viability over 48h, with no observed cytotoxicity.
Conclusions:
- The processed phospholipoproteic secretome-derived formulation exhibits preserved compositional stability and analytical reproducibility.
- Short-term ex vivo cellular compatibility is maintained across evaluated processing and storage conditions.
- The study provides a quantitative framework for evaluating such preparations, with future studies needed for functional and long-term effects.
