Biomonitoring of Occupational Exposure to Mycotoxins Among Swine Farm Workers: An Italian Pilot Study

The risk of exposure to mycotoxins in livestock farming is still poorly characterized, particularly in Italy where human biomonitoring data are scarce. Livestock farms represent a high-risk setting due to frequent handling of contaminated feed and dust-generating activities. This pilot study applied a human biomonitoring approach to assess internal exposure to multiple mycotoxins among pig farmers in Southern Italy. Urinary biomarkers of aflatoxin B1 (AFB1), aflatoxin M1 (AFM1), ochratoxin A (OTA), and fumonisin B1 (FB1), together with oxidative stress biomarkers (8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), 3-nitrotyrosine (3-NO₂Tyr), and 5-methylcytidine (5-MeCyt)), were measured in urine samples from 35 workers and 30 non-exposed controls. A sensitive and validated HPLC-MS/MS multi-mycotoxin method was developed and applied. Biomonitoring results were also discussed in relation to previous environmental monitoring. AFM1 emerged as the most frequently detected biomarker in the exposed group, with concentrations above the limit of detection (LOD) in 22.8% of samples; 11.4% exceeded the limit of quantification (LOQ). In contrast, only 10% of the control samples had values above the LOD and none exceeded the LOQ, suggesting a possible contribution linked to occupational tasks. This study provides original biomonitoring evidence of low-dose, mixed mycotoxin exposure among Italian swine farmers and highlights the value of integrating environmental and biological monitoring to improve occupational exposure assessment in livestock production systems.