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LncRNA ABCA9-AS1 as a ceRNA Promotes Gastric Cancer Progression by Sponging miR-497-5p to Regulate KIF23 Expression
Yiwen Wu1, Yiniu Xia1, Hanyu Liu1
1Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan Province, China.
Current Cancer Drug Targets
|July 28, 2026
Summary
Long noncoding RNA ABCA9-AS1 promotes gastric cancer progression by increasing KIF23 expression and enhancing cell proliferation and metastasis. This study reveals ABCA9-AS1 as a potential biomarker for gastric cancer.
Area of Science:
- Molecular Oncology
- Cancer Biology
- RNA Biology
Background:
- Long noncoding RNAs (lncRNAs) are key players in cancer pathology.
- The role of lncRNA ABCA9-AS1 in gastric cancer remains unexplored.
Purpose of the Study:
- Investigate the impact of lncRNA ABCA9-AS1 on gastric cancer cell proliferation and metastasis.
- Elucidate the involvement of the miR-497-5p/KIF23 signaling pathway.
Main Methods:
- Constructed a gastric cancer-specific competing endogenous RNA (ceRNA) network using TCGA data.
- Utilized qRT-PCR, siRNA knockdown, and plasmid overexpression to analyze gene expression and function.
- Assessed cell proliferation, migration, and invasion using CCK-8, colony formation, Transwell, and Matrigel assays.
Main Results:
- lncRNA ABCA9-AS1 was significantly upregulated in gastric cancer cells, with decreased miR-497-5p and increased KIF23.
- ABCA9-AS1 knockdown inhibited proliferation, migration, and invasion, effects reversed by KIF23 restoration.
- ABCA9-AS1 functions as a ceRNA, sponging miR-497-5p to upregulate KIF23.
Conclusions:
- lncRNA ABCA9-AS1 is aberrantly upregulated in gastric cancer and promotes tumor progression.
- The ABCA9-AS1/miR-497-5p/KIF23 axis is a critical regulator of gastric cancer cell behavior.
- ABCA9-AS1 is a promising prognostic biomarker for gastric cancer.
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