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The Combination of Mechanically Isolated Stromal Vascular Fraction and Fibrin Hydrogel: A Processing Protocol
Published on: November 17, 2023
Shelf-Stable, Rapid-Gelling, and Robust Thrombin-Independent Fibrinogen-Based Hydrogels for Massive Hemorrhage
Yihang Ding1, Zhaodi Liu2,3, Xizhuo Jing1
1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.
Advanced Healthcare Materials
|July 28, 2026
Summary
A new fibrinogen-based hydrogel (GMK/FM) offers rapid and stable hemostasis for trauma care. This advanced bioadhesive shows superior strength and adhesion, significantly reducing blood loss in animal models.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Emergency Medicine
Background:
- Effective hemorrhage control is critical in trauma and emergency medicine.
- Current bioadhesives face limitations in mechanical strength, biocompatibility, and storage.
- There is a need for advanced hemostatic agents with improved clinical applicability.
Purpose of the Study:
- To develop a novel thrombin-independent fibrinogen-based hydrogel for rapid and effective hemostasis.
- To enhance mechanical properties, tissue adhesion, and storage stability of bioadhesives.
- To evaluate the hemostatic performance and biocompatibility of the developed hydrogel.
Main Methods:
- Fabrication of a hydrogel from knob-peptide-grafted gelatin methacryloyl (GMK) and fibrinogen methacryloyl (FM).
- Utilized knob-hole crosslinking followed by photo-crosslinking for hydrogel reinforcement.
- Assessed gelation time, mechanical strength, tissue adhesion, and storage stability.
- Evaluated hemostatic efficacy in rat liver and abdominal aorta hemorrhage models.
Main Results:
- The GMK/FM hydrogel exhibited rapid gelation (< 2 s) and robust mechanical strength.
- Demonstrated enhanced tissue adhesion and superior storage stability (4 weeks at room temperature) compared to fibrin glue.
- Significantly reduced hemostatic time and blood loss in severe hemorrhage models.
- Showcased excellent biocompatibility in preclinical evaluations.
Conclusions:
- The developed GMK/FM hydrogel presents a promising hemostatic agent with rapid action, enhanced properties, and improved stability.
- Its strong performance in preclinical models suggests significant potential for clinical applications in trauma and emergency medicine.
- This novel bioadhesive addresses key limitations of existing treatments, offering a viable solution for critical bleeding management.
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