Circulating Circ_0089762 Predicts Adverse Outcomes in Acute Myocardial Infarction Via Regulating...

Area of Science:

• Cardiovascular Biology
• Molecular Medicine
• Biomarker Discovery

Background:

• Endothelial dysfunction initiates atherosclerosis and persists in acute myocardial infarction (AMI).
• Circular RNAs (circRNAs) play a critical regulatory role in cardiovascular diseases, including AMI.

Purpose of the Study:

• To investigate the expression and clinical significance of circ_0089762 in AMI.
• To elucidate the molecular mechanism of circ_0089762 in regulating hypoxia/reoxygenation (H/R)-induced damage in human umbilical vein endothelial cells (HUVECs).

Main Methods:

• Serum levels of circ_0089762, miR-938, and STAT3 were quantified using RT-qPCR.
• An H/R injury model in HUVECs was established to assess the effects of modulating circ_0089762 and miR-938 on cell viability, apoptosis, migration, oxidative stress, and inflammation.
• Dual-luciferase assays and rescue experiments were employed to validate targeting relationships and the regulatory axis.

Main Results:

• circ_0089762 was found to be hyper-expressed in AMI patients, demonstrating diagnostic accuracy and association with major adverse cardiovascular events (MACE).
• H/R treatment upregulated circ_0089762 expression; silencing circ_0089762 improved endothelial cell function and reduced inflammatory markers.
• circ_0089762 directly targets miR-938, which in turn targets STAT3, forming a regulatory axis.

Conclusions:

• circ_0089762 exacerbates H/R-induced endothelial cell injury by regulating the miR-938/STAT3 pathway.
• circ_0089762 shows potential as a biomarker for AMI diagnosis and prognosis, and as a target for therapeutic intervention.