Step-by-Step Technique for Platelet-Rich Plasma Gel Preparation and Use in Knee Osteoarthritis

Knee osteoarthritis (OA) remains a leading cause of pain and disability worldwide, and current injectable options such as corticosteroids and hyaluronic acid often provide limited or short-term benefits. Orthobiologic therapies, particularly platelet-rich plasma (PRP), have gained attention for their regenerative and anti-inflammatory potential. PRP plasma gel is a autologous formulation obtained through controlled thermal denaturation of plasma proteins, producing an albumin-based semisolid matrix subsequently mixed with fresh PRP. This process results in a homogeneous gel capable of sustained release of bioactive growth factors, potentially prolonging intra-articular residence time and clinical effect compared with conventional PRP. We describe a step-by-step technique for preparing PRP plasma gel using standard laboratory equipment, without the need for commercial kits. The method is reproducible, low cost, and suitable for outpatient settings, including resource-limited environments. Clinical application is shown in knee osteoarthritis, where the formulation can be injected intra-articularly as an accessible and versatile orthobiologic option. The technique offers technical and economic advantages, with the potential to broaden the use of orthobiologic therapies in daily practice.