Related Experiment Video
Updated: Aug 5, 2026

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
Published on: February 9, 2021
Synthesis and Biological Evaluation of Aryl(Benzofuran-5-yl)-[1,2,4]Triazolo[4,3-a]Pyridine Derivatives as Anticancer
Raju Veeramalla1,2, Savita Belwal1, Laxminarayana Eppakayala1
1Department of Chemistry, Anurag University, Hyderabad, India.
Abstract:
A new library of aryl(benzofuran-5-yl)-[1,2,4]triazolo[4,3-a]pyridines (12a-j) has been synthesized, and their chemical structures were confirmed by 1H NMR, 13C NMR, and mass spectral data. Further, these derivatives were assessed against four human cancer cell lines, including human prostate cancer (PC3), human lung cancer (A549), human breast cancer (MCF-7), and human ovarian cancer (A2780) by using the MTT method. Among the tested compounds, five compounds, 12a-12e, showed the most potent activity. Particularly, one compound, 12a, possessed the most promising activity.
Related Concept Videos
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
Five-Membered Heterocyclic Aromatic Compounds: Overview
Diazonium Group Substitution with Halogens and Cyanide: Sandmeyer and Schiemann Reactions
Nucleophilic Aromatic Substitution of Aryldiazonium Salts: Aromatic SN1
In the Sandmeyer reaction, for example, the diazonio group is replaced by a chloro, bromo, or cyano...
Electrophilic Aromatic Substitution: Fluorination and Iodination of Benzene
Physical Properties of Amines
