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Updated: Aug 5, 2026

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Published on: October 5, 2016
Low-frequency polarized electric field promotes stilbene biosynthesis by redirecting phenylpropanoid flux in
Jie Zhu1, Huangqiao Tao2, Shi Chen2
1Department of Food Nutrition and Safety/National R&D Center for Chinese Herbal Medicine Processing, College of Engineering, China Pharmaceutical University, Nanjing, 211198, China; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
Abstract:
Resveratrol is a health-promoting stilbenoid in grape berries, but its postharvest accumulation remains limited. Here, grape berries were treated with a low-frequency polarized electric field (LFPEF) at 2500 V and 50 Hz for 3 h/d during storage. LFPEF increased trans-resveratrol content from 3.05 to 4.42 mg/kg FW after 12 d, corresponding to an approximately 1.45-fold enhancement. Integrated metabolomic and transcriptomic analyses showed that LFPEF redirected phenylpropanoid metabolism from anthocyanin accumulation toward stilbene biosynthesis, accompanied by increased precursor availability, enhanced STS activity and VvSTS expression, and reduced CHS-associated branch activity. VvWRKY40 was identified as an LFPEF-responsive regulator that bound to W-box elements in the VvSTS1 promoter and activated its transcription. LFPEF enhanced VvWRKY40 protein abundance, whereas VvWRKY40 silencing attenuated LFPEF-induced VvSTS expression and resveratrol accumulation. These findings support LFPEF as a non-thermal strategy to improve the functional quality of postharvest grapes.
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