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Aging time-dependent non-volatile chemical and microbial dynamics in Pericarpium Citri Reticulatae 'Chachiensis' from
Shuting Huang1, Wenfeng Wang1, Jie Xu1
1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, China.
Abstract:
Pericarpium Citri Reticulatae 'Chachiensis' (PCRC), a renowned traditional Chinese medicine, exhibits enhanced efficacy following prolonged aging, yet the underlying mechanisms remain poorly understood. This study employed metabolomics and microbiomics to analyze multi-source PCRC, elucidating the dynamic changes in non-volatile chemicals and microbial communities over a 7-year aging period. In the metabolomic analysis, a total of 306 differential metabolites were identified as potential indicators of aging, among which seven metabolites exhibited a consistent time-dependent increase in relative content over the seven-year period. Microbiomic analysis revealed that Bacillus and Xeromyces were significantly enriched in 7-year-aged PCRC and consistently dominated the microbial community throughout the aging. Spearman correlation analysis indicated a potential association between dominant microorganisms and aging time-dependent metabolites, suggesting that their interaction was a critical component of the PCRC aging process. These findings provide novel insights into PCRC aging mechanisms and establish a theoretical foundation for quality assessment and aging time classification.
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