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Influence of nicotine on protein expression around hydrophilic osseointegrated implants: A proteomic study in male
Karoline Bernardes do Nascimento1, Bruno Luís Graciliano Silva1, Julio Cesar Sánchez-Puetate2
1Department of Diagnosis and Surgery, Sao Paulo State University, Araraquara School of Dentistry, Araraquara, Sao Paulo, Brazil.
Objective:
To ensure the success of dental implant treatment, various factors must be considered, including osseointegration and systemic conditions. There is evidence in the literature that smokers may exhibit alterations in tissue healing, which can compromise the success of implant rehabilitation. Therefore, this study aimed to investigate the influence of nicotine on the protein profile of bone tissue around hydrophilic implants during the osseointegration process in rats.
Design:
Bone tissue samples from the control and nicotine groups (n = 3 per group) were subjected to protein extraction, mass spectrometry, and bioinformatic analyses. Protein identification was performed using Proteome Discoverer 2.1 software and the SEQUEST algorithm, and the protein data were compared with those of a protein database of Rattus norvegicus obtained from UniProt.
Results:
A total of 740 proteins were detected in both the control group and the nicotine-exposed group. Among them, the proteins biglycan, periostin and histone H4 were highlighted because of their higher abundance in the healthy implant group, while they were reduced in the nicotine-exposed group.
Conclusions:
Nicotine has the potential to alter the protein profile of bone tissue around hydrophilic implants during osseointegration, which may impair tissue remodeling and healing.
