Development and Optimization of Antioxidant Poly Herbal Peel-off Mask using Doe Based Approach

Area of Science:

• Cosmetic Science
• Dermatology
• Natural Product Chemistry

Background:

• Antioxidants combat free radical damage, mitigating skin aging signs like wrinkles and pigmentation.
• Combining multiple antioxidants in cosmetic formulations can enhance protective effects.
• This research explores a polyherbal mask leveraging Ocimum sanctum, Moringa oleifera, and Trigonella foenum-graecum extracts for antioxidant benefits.

Purpose of the Study:

• To formulate and optimize a polyherbal antioxidant peel-off mask.
• To evaluate the physicochemical properties, in vitro antioxidant capacity, and skin irritation potential of the mask.

Main Methods:

• Central Composite Design (CCD) was employed for formulation optimization.
• Polyvinyl alcohol (PVA) and carbomer 940 were used as film-forming agents.
• Physicochemical analysis, in vitro antioxidant assays, and the HET-CAM assay for irritation were conducted.

Main Results:

• The optimized mask demonstrated a drying time of 25.3 minutes and good applicability.
• Physicochemical evaluations confirmed homogeneity, smooth texture, and suitable pH for skin application.
• In vitro antioxidant assays revealed superior efficacy compared to Vitamin C, with no observed irritation in the HET-CAM test.

Conclusions:

• A polyherbal peel-off mask with effective in vitro antioxidant activity and favorable physicochemical properties was successfully developed.
• Central Composite Design facilitated efficient optimization of the formulation.
• The formulation shows promise for cosmetic skincare, pending further in vivo and stability assessments.