An experimental test of trait-based restoration to achieve drought tolerance and invasion resistance in two...

Drought and invasive species are threats to primary productivity in grassland ecosystems worldwide. A central challenge in ecological restoration is establishing plant communities that can withstand these abiotic and biotic stressors. We tested the efficacy of a trait-based framework for enhancing drought tolerance and invasion resistance in experimental restorations of contrasting grassland systems, a perennial mixed-grass prairie in Wyoming and annual interior grassland in California. Each experiment included four trait-based seeding treatments: drought tolerant, invasion resistant, functionally diverse, and a random control. All seeded communities were subjected to extreme precipitation reduction (-50% annual) and invasion by non-native annual grasses. We asked two questions: (1) Can we establish communities from seed that meet and maintain these desired trait-based targets? (2) Did the trait-based seeding treatments tolerate extreme precipitation reduction or resist invasion by non-native annual grasses? Based on analyses of compositional dissimilarities, we found trait-based restoration targets were more difficult to maintain in an annual-dominant grassland rather than in a perennial-dominant one, and targets composed of multiple community-weighted mean traits were more difficult to meet than targets that maximized functional diversity. In both grasslands, plant communities with drought-tolerant traits maintained growth rates under reduced precipitation, but these effects diminished after multiple years of drought and during drought release. This highlights the importance of including a diversity of strategies when restoring plant communities. Our proposed invasion-resistant traits did not consistently reduce non-native annual grass establishment, but communities with traits that conferred drought tolerance were the most effective at resisting invasion, suggesting that similar traits enhance drought tolerance and resist invasion in these grasslands. Our findings indicate that restored plant communities with high functional diversity may be able to respond to variable conditions better than communities with traits designed to meet specific restoration objectives. The results from this restoration experiment suggest that our understanding of the traits underlying drought tolerance is better than our understanding of the traits underlying invasion resistance. However, if drought tolerance enhances competitive ability in arid and semiarid grassland ecosystems, then physiological theories of resource use can be applied to enhance invasion resistance.