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Updated: Aug 5, 2026
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Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles
Published on: August 28, 2015
Formulation, Characterization, and Preliminary In Vitro Evaluation of Letrozole Incorporated Eudragit® RS 100
Muhammet Ali Polat1, Kadir Aykaç2,3, Zerrin Cantürk4
1Anadolu University, Institute of Graduate Education, 26470, Eskisehir, Türkiye.
Current Pharmaceutical Design
|July 29, 2026
Summary
This study developed letrozole-loaded polymeric nanoparticles for hepatocellular carcinoma therapy. The nanoparticles showed sustained drug release and maintained cytotoxic activity, indicating potential for HCC treatment.
Area of Science:
- Nanotechnology
- Materials Science
- Oncology
Background:
- Hepatocellular carcinoma (HCC) presents a significant therapeutic challenge.
- Effective drug delivery systems are crucial for improving HCC treatment outcomes.
- Letrozole (LTZ) has shown potential anticancer properties but requires optimized delivery.
Purpose of the Study:
- To formulate and characterize letrozole (LTZ)-loaded polymeric nanoparticles (PNPs) using Eudragit® RS100.
- To evaluate the potential of these PNPs as a drug delivery system for hepatocellular carcinoma (HCC).
Main Methods:
- Polymeric nanoparticles were prepared using the spray-drying technique.
- Characterization included morphology (SEM), particle size, PDI, zeta potential, drug loading, thermal analysis, and in vitro release studies.
- Cytotoxicity was assessed using the MTT assay on HepG2 and BJ cell lines.
Main Results:
- Spherical nanoparticles (253-425 nm) with positive zeta potential (36-48 mV) and good encapsulation efficiency (47.9%-56.9%) were successfully prepared.
- In vitro studies showed an initial burst release followed by sustained LTZ release (~80% over 24 h) at pH 7.4.
- Nanoparticles preserved LTZ's cytotoxic activity against HepG2 cells, with an IC50 > 121.85 µg/mL.
Conclusions:
- LTZ-loaded PNPs were successfully developed with favorable physicochemical properties and sustained release.
- The nanoparticles demonstrate potential as an effective drug delivery system for HCC therapy.
- Further in vivo studies are warranted to confirm therapeutic efficacy.
