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[Development and application of a method for quantifying plasma cell-free DNA concentration based on Spike-in

Xiong Yang1,2, Changlin Yang2,3, Guodan Zeng2,3

  • 1Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, College of Life and Geographic Sciences, Kashi University, Kashi 844000, Xinjiang, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|July 29, 2026
PubMed
Summary

This study introduces a new method to measure cell-free DNA (cfDNA) using whole-genome bisulfite sequencing (WGBS) data, simplifying quantification for large studies.

Keywords:
DNA methylationSpike-in quantificationcell-free DNA concentrationhigh-throughput sequencingtissues-of-origin of cell-free DNA

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