Related Experiment Video

Updated: Aug 5, 2026

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
07:38

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Published on: May 17, 2010

Live-cell PAINT microscopy of protein targets via fluorogenic exchangeable HaloTag ligands

Tong Zhan, Nels C Gerstner, Julia G Martin

    Biorxiv : the Preprint Server for Biology
    |July 29, 2026
    PubMed

    More Related Videos

    Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates06:48

    Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates

    Published on: January 5, 2024

    Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells11:21

    Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

    Published on: June 26, 2010

    Related Concept Videos

    Labeling DNA Probes03:31

    Labeling DNA Probes

    DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
    Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
    Protein Dynamics in Living Cells01:19

    Protein Dynamics in Living Cells

    Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
    Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
    Tagging and Fusion Proteins01:24

    Tagging and Fusion Proteins

    Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...