Related Experiment Video
Updated: Aug 5, 2026
07:16
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor
Published on: September 13, 2018
Proteomic Analysis of Hippo Pathway Protein-Protein Interactions
1Department of Developmental and Cell Biology, University of California, Irvine, Irvine, California 92697, USA.
Cold Spring Harbor Perspectives in Biology
|July 29, 2026
Summary
The Hippo pathway, crucial for organ size and development, is better understood through mass spectrometry (MS)-based proteomics. This approach maps protein-protein interactions (PPIs), advancing Hippo pathway research and therapeutic development.
Area of Science:
- Cell Biology
- Biochemistry
- Proteomics
Background:
- The Hippo pathway regulates crucial biological processes like organ size and tissue homeostasis.
- Protein-protein interactions (PPIs) are central to understanding Hippo pathway signaling.
- Mass spectrometry (MS)-based proteomics has revolutionized interactome mapping.
Purpose of the Study:
- To review MS-based biochemical approaches for analyzing PPIs.
- To summarize the progress in characterizing Hippo pathway PPIs.
- To highlight the utility of the Hippo interactome in signaling and disease research.
Main Methods:
- Utilizing mass spectrometry (MS) for proteomics.
- Employing biochemical approaches to analyze protein-protein interactions (PPIs).
- Mapping the Hippo pathway interactome.
Main Results:
- MS-based proteomics has expanded the known regulatory and functional landscapes of the Hippo pathway.
- Interactome mapping provides insights into Hippo pathway signaling.
- The Hippo pathway serves as a model for MS-driven biological discovery.
Conclusions:
- MS-driven interactome analysis significantly accelerates biological research.
- Understanding Hippo pathway PPIs is key for future therapeutic development.
- The Hippo pathway is a valuable model for demonstrating the power of proteomics.
Related Concept Videos
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
02:04Protein-protein Interfaces
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...