Related Experiment Video
Updated: Aug 5, 2026

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
c-Myc-Regulated lncRNA AFAP1-AS1 Promotes Proliferation of Oral Squamous Cell Carcinoma via the miR-655-3p/CCNA2 Axis
Panpan Liu1,2, Qian Wu3, Tao Li2,4
1Department of Pediatric Dentistry, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, China.
Objective:
This study aimed to investigate the molecular mechanisms by which AFAP1-AS1 promotes proliferation in oral squamous cell carcinoma (OSCC).
Materials And Methods:
AFAP1-AS1 expression was examined in OSCC tissues and cell lines. Functional analyses were performed by silencing AFAP1-AS1 in vitro and assessing the resulting changes in CCNA2 expression. Bioinformatic analyses were used to predict microRNAs (miRNAs) potentially interacting with both AFAP1-AS1 and CCNA2, and these interactions were validated using dual-luciferase reporter assays and RNA immunoprecipitation (RIP). Chromatin immunoprecipitation (ChIP) assays were conducted to determine whether c-Myc directly binds to the AFAP1-AS1 promoter, thereby regulating its transcription.
Results:
AFAP1-AS1 was significantly upregulated in OSCC tissues and cell lines compared with normal controls. Silencing AFAP1-AS1 led to decreased CCNA2 expression. Both computational predictions and experimental validations indicated that miR-655-3p interacts with AFAP1-AS1 and CCNA2, potentially acting as a regulatory intermediary. ChIP assays further showed that c-Myc directly binds to the promoter region of AFAP1-AS1, suggesting a role in its transcriptional activation.
Conclusion:
These findings suggest that c-Myc-regulated AFAP1-AS1 may contribute to OSCC proliferation through modulation of the miR-655-3p/CCNA2 axis and provide insight into the molecular mechanisms underlying OSCC progression.
Related Concept Videos
Abnormal Proliferation
Induced Pluripotent Stem Cells
Somatic cells are...
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
lncRNA - Long Non-coding RNAs
Cancer Stem Cells and Tumor Maintenance
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...