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Decoding strain level interactions between Vibrio cholerae and gut microbiota
Guozhong Chen1,2, Shucheng Li3, Qinghua Zhao4
1Department of Basic Medical Sciences, The 960th Hospital of PLA, Jinan, Shandong, China.
Virulence
|July 30, 2026
Summary
Gut bacteria strains significantly impact Vibrio cholerae
Area of Science:
- Microbiology and Gut Microbiome Research
Background:
- Vibrio cholerae causes severe diarrheal disease.
- Gut microbiota and their byproducts influence V. cholerae colonization.
- Strain-level effects of gut bacteria on V. cholerae physiology are understudied.
Purpose of the Study:
- To systematically investigate the strain-specific effects of gut bacterial culture supernatants on V. cholerae physiology.
- To identify specific gut bacterial strains that can modulate V. cholerae growth, biofilm formation, motility, and gene expression.
- To validate potential probiotic candidates for cholera prevention and treatment.
Main Methods:
- Systematic screening of various gut bacterial culture supernatants.
- Assays to measure V. cholerae growth inhibition, biofilm formation, motility, and reactive oxygen species (ROS) resistance.
- Analysis of V. cholerae gene expression modulation.
- Integration of findings with human cholera cohort data.
Main Results:
- Specific gut bacterial supernatants inhibited V. cholerae growth (e.g., Clostridium butyricum, Ruminococcus bromii, Bacteroides clarus).
- Other supernatants enhanced V. cholerae biofilm formation (e.g., Akkermansia muciniphila, Roseburia inulinivorans).
- Gut bacteria modulated V. cholerae motility and ROS resistance (linked to KatE activity in Eubacterium rectale and R. inulinivorans).
- Modulation of V. cholerae regulatory gene expression was observed.
- Ruminococcus bromii CNGBCC 1800554 was identified as a promising probiotic candidate.
Conclusions:
- Gut bacterial culture supernatants exert diverse and profound strain-specific effects on V. cholerae physiology.
- This study provides a foundation for understanding gut microbiota-V. cholerae interactions at the strain level.
- Identified bacterial strains and Ruminococcus bromii offer potential for developing next-generation probiotics for cholera management.
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