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Sugammadex interference with serum progesterone measurement: an in-vitro study
Tamblyn Devoy1,2, Theresa Larkin3, Miles Allan2
1Department of Anaesthesia, Wollongong Hospital, Wollongong, NSW, Australia.
Anaesthesia
|July 30, 2026
Summary
Sugammadex can interfere with progesterone tests, leading to inaccurate results in women. Laboratory progesterone measurements require caution when sugammadex is present.
Area of Science:
- Pharmacology
- Clinical Chemistry
- Analytical Chemistry
Background:
- Sugammadex, a reversal agent for neuromuscular blockade, may affect laboratory test results.
- Interference with immunoassays can lead to misinterpretation of hormone levels.
Purpose of the Study:
- To investigate the impact of sugammadex on serum progesterone measurements.
- To determine if sugammadex causes interference in routine immunoassay testing for progesterone.
Main Methods:
- Serum samples from pre-menopausal women were spiked with varying sugammadex concentrations (15-120 μM).
- Progesterone levels were measured using electrochemiluminescence immunoassay and liquid chromatography-tandem mass spectroscopy.
- Comparison of results between baseline and sugammadex-spiked samples.
Main Results:
- Sugammadex demonstrated a dose-dependent reduction in measured progesterone levels via immunoassay.
- A mean decrease of 34.6% in progesterone was observed at 120 μM sugammadex.
- Liquid chromatography-tandem mass spectroscopy showed no significant change in progesterone concentration.
Conclusions:
- Sugammadex interferes with immunoassay-based progesterone testing, causing falsely decreased results.
- This interference is clinically relevant at typical sugammadex doses.
- Progesterone test results should be interpreted cautiously in patients receiving sugammadex.