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Updated: Aug 5, 2026

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07:56

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Protein Extraction From Mycobacterium spp. for Identification by MALDI-TOF Mass Spectrometry

Lucas Evangelista Marques1, Stephanie Chrystine Balestro Mota1, Christoffel Opperman2,3,4

  • 1Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, Brazil.

Current Protocols
|July 30, 2026
PubMed
Summary

This study presents a standardized protocol for extracting proteins from Mycobacterium species for identification using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). The method ensures reliable species-level discrimination in diagnostic and research settings.

Keywords:
MALDI‐TOFMycobacteriummass spectrometryprotein extractionsample preparation

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