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Updated: Aug 5, 2026
Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion
Published on: September 1, 2018
Protocol for induction and quantification of injury-primed axon outgrowth in adult mouse DRG neurons
Eliana Seburn1, Brett J Hilton1
1Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC V5Z 1M9, Canada; International Collaboration on Repair Discoveries (ICORD), University of British Columbia, Vancouver, BC, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, BC, Canada.
Abstract:
Adult dorsal root ganglion (DRG) neurons provide a powerful model to study axon growth. Dissociated lumbar L3-L5 DRG neurons extend axons de novo in culture, enabling the measurement of baseline outgrowth. Here, we present a protocol for inducing and quantifying injury-primed axon outgrowth in adult mouse DRG neurons. We describe steps for sciatic nerve conditioning injury, isolating and culturing injured L3-L5 DRG neurons, and quantifying axon outgrowth. This protocol is compatible with cytoskeletal imaging and molecular profiling of injury-induced changes.
