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Sperm morphology, functionality, and cryopreservation challenges in wild Ocellate River stingrays Potamotrygon motoro
Nathalia Dos Santos Teixeira1, Jhony Lisbôa Benato2, Rômulo Batista Rodrigues3
1Veterinary Research Program, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Tissue & Cell
|July 30, 2026
Summary
Cryopreservation challenges for Ocellate River stingray (Potamotrygon motoro) sperm were studied. Unique helical sperm structures and aggregation impact cryopreservation success, requiring specialized protocols for freshwater stingrays.
Area of Science:
- Ichthyology
- Reproductive Biology
- Cryobiology
Background:
- The Ocellate River stingray (Potamotrygon motoro) is a freshwater elasmobranch species.
- Understanding reproductive biology is crucial for conservation efforts.
Purpose of the Study:
- To characterize sperm morphology and morphometry in wild Potamotrygon motoro.
- To evaluate the effects of cryopreservation on sperm integrity and viability.
Main Methods:
- Semen collection from mature male stingrays.
- Scanning electron microscopy for ultrastructural analysis.
- Motility analysis and membrane integrity assessment post-cryopreservation.
Main Results:
- Potamotrygon motoro sperm exhibit a helical head structure and form spermatozeugmata.
- Cryopreservation increased morphological defects, particularly in midpiece and tail regions.
- Post-thaw membrane integrity was low (1.5%-7.9%); 10% DMSO and 10% DMF showed highest motility retention.
Conclusions:
- The unique sperm ultrastructure and aggregation of Potamotrygon motoro present significant challenges for cryopreservation.
- Optimizing cryopreservation protocols is essential for the effective conservation of freshwater stingrays.
- Further research is needed to overcome cryoinjury sensitivity in these species.
