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Association of initial interface formation time with CD34+ collection efficiency in continuous mononuclear cell
Hiroyuki Murakami1, Keiko Fujii2, Wataru Kitamura1
1Department of Hematology, Okayama University Hospital, Okayama, Japan; Division of Transfusion and Cell Therapy, Okayama University Hospital, Okayama, Japan; Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
The time taken to establish a stable interface during mononuclear cell collection is linked to CD34+ cell collection efficiency. Longer interface formation times correlate with lower collection efficiency, impacting stem cell harvesting outcomes.
Area of Science:
- Hematology
- Cellular Biology
- Medical Technology
Background:
- Peripheral blood stem cell (PBSC) harvesting efficiency is influenced by various factors, but procedural management's impact remains understudied.
- Continuous mononuclear cell (CMNC) collection relies on a stable cell-plasma interface (IF) for optimal performance.
- While IF instability is known to reduce collection efficiency (CE), the adequacy of initial IF formation as an independent factor has not been thoroughly examined.
Purpose of the Study:
- To investigate the association between initial interface formation time (IFT) and CD34+ cell collection efficiency (CE) in CMNC procedures.
- To determine if IFT is an independent predictor of CE during apheresis.
- To explore the early dynamics of IF formation and its impact on stem cell collection outcomes.
Main Methods:
- Retrospective analysis of 291 Spectra Optia CMNC procedures (autologous and allogeneic).
- Definition of Initial Interface Formation Time (IFT) as the duration from procedure start to MNC collection initiation.
- Calculation of Collection Efficiency 2 (CE2) based on collected CD34+ cells and pre-apheresis values.
Main Results:
- CE2 was significantly lower in autologous (41.3%) compared to allogeneic (59.2%) collections.
- Longer IFT (median 18 min) was associated with lower CE2 compared to shorter IFT (median 15 min).
- CE2 correlated inversely with IFT (r=-0.23) and white blood cell count (r=-0.24), and positively with hematocrit (r=0.36) (all p<0.001). IFT was inversely correlated with hematocrit (r=-0.44, p<0.001).
Conclusions:
- Initial interface formation time (IFT) is an independent factor associated with CD34+ cell collection efficiency (CE2).
- IFT offers valuable insights into the early procedural dynamics of interface formation during leukapheresis.
- Optimizing initial IF formation may be a key procedural aspect to enhance stem cell collection efficiency.
