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Updated: Aug 5, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
Published on: May 23, 2018
Microbial single-cell sequencing technologies: Principles, applications, and spatial integration
Fei Xu1, Fangyu Mo1, Babatunde O Kehinde2
1Hainan Institute, Zhejiang University, Sanya 572025, China; Institute of Crop Science & Institute of Bioinformatics, Zhejiang University, Hangzhou 310058, China.
Abstract:
The advent of microbial single-cell sequencing technology provides unprecedented resolution to study microbial ecosystems, revealing cellular heterogeneity, interactions, and genetic evolution of microorganisms. This review systematically compiles current microbial single-cell sequencing technologies, providing a detailed synthesis of their underlying technical principles, illuminating the strengths and limitations of various approaches. In-depth summaries of the technical challenges they encounter in different microbial domains and their practical applications are provided. Finally, we summarized the emerging field of microbial spatial omics, with a particular focus on advanced imaging techniques utilizing sequencing and fluorescence in situ hybridization.
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