Development of a Novel Immunohistochemistry Assay to Characterize Extradomain-B of Fibronectin (EDB+FN) Protein...

Tumor stroma, the cellular and noncellular support system surrounding cancer cells, is a promising source of novel therapeutic targets. Extradomain-B of fibronectin (EDB+FN), a tumor-specific antigen expressed in the extracellular matrix of tumor stroma and minimally in healthy tissues, is the target of the first-in-concept antibody-drug conjugate micvotabart pelidotin, which is currently in clinical trials (NCT05720117 and NCT06795412) for the treatment of solid tumors. A specific and reproducible immunohistochemistry (IHC) assay and scoring approach are needed to detect EDB+FN protein expression for indication selection, preclinical research, and potential future evaluation of correlations between target expression and therapeutic efficacy.

An EDB+FN IHC assay was developed to detect EDB+FN protein in human solid tumors. A novel scoring system was developed, adapting the H-score methodology to evaluate the intensity and localization of EDB+FN extracellularly in tumor stroma and cellularly in cancer cells in 10 solid tumor types. Moreover, a custom, artificial intelligence-powered digital pathology algorithm was developed to streamline scoring of EDB+FN protein expression for research and future analysis of spatial distribution.

The developed IHC assay reproducibly detected EDB+FN in the tumor stroma across formalin-fixed paraffin-embedded samples from 10 tumor types and minimally in normal tissue. Percent stroma was not predictive of EDB+FN expression within a tumor, suggesting heterogeneous EDB+FN protein expression in tumor stroma. The modified H-scoring approach reproducibly quantified EDB+FN expression extracellularly in tumor stroma by pathologist evaluation, and a digital algorithm built with open-source tools demonstrated proof-of-concept for digital quantification of EDB+FN protein expression.

This study demonstrates technical characterization of a research-use IHC assay for detection of EDB+FN in formalin-fixed paraffin-embedded tumor sections, reveals broad expression of EDB+FN across cancer types, and establishes a novel approach for scoring expression of a non-cell-associated protein in the tumor extracellular matrix, both by pathologists and using digital pathology.