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Bis-Tetrazine Fluorogenic (Silicon)-Rhodamine Dyes for Live-Cell Labeling
Sabrina Giofrè1,2, Lukas Schartel1,3, Richard Wombacher4
1Biocenter, Johannes Gutenberg University Mainz, 55128Mainz, Germany.
Journal of the American Chemical Society
|July 30, 2026
Summary
Researchers developed new fluorogenic click dyes for biorthogonal labeling. These dyes offer significantly enhanced fluorescence turn-on, improving live-cell imaging and studies of protein dynamics.
Area of Science:
- Chemical Biology
- Organic Chemistry
- Biochemistry
Background:
- Fluorogenic click dyes are essential for real-time biomolecule visualization.
- Efficient quenching and high fluorescence turn-on in a single dye remain challenging.
Purpose of the Study:
- To develop novel fluorogenic click dyes with improved performance.
- To explore their utility in live-cell imaging and protein dynamics studies.
Main Methods:
- Structural modification of (silicon)-rhodamine dyes at the xanthene scaffold.
- Synthesis of mono- and bis-functional derivatives.
- Evaluation of fluorogenic response and application in peptide cyclization.
Main Results:
- Developed dyes with fluorescence enhancements up to 2 orders of magnitude (monofunctional) and 3 orders of magnitude (bis-functional).
- Bis-functional dyes are suitable for live-cell applications and anisotropy measurements.
- Demonstrated utility in peptide cyclization for enhanced cellular uptake and visualization.
Conclusions:
- Introduced a versatile class of fluorogenic click dyes with superior performance.
- These dyes significantly expand the capabilities of click chemistry for live-cell imaging and protein studies.
