Related Experiment Video
Updated: Aug 5, 2026
08:02
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence
Published on: March 12, 2020
A Fluorescent Probe for Carboxylesterases Detection in Estradiol Valerate-Treated Inflammation Alleviation
Wenqiang Jiang1, Zhenhua Zhong1, Lubing Tang1
1Department of Breast Surgery, Womens and Childrens Hospital Affiliated to Ningbo University, Ningbo, 315000, China.
Journal of Fluorescence
|July 30, 2026
Summary
A new fluorescent probe, PIPM-CE, enables precise detection of carboxylesterases (CE) for monitoring inflammation alleviation during estradiol valerate (EV) therapy. This allows for optimized treatment and prevention of adverse effects.
Area of Science:
- Analytical Chemistry
- Biomedical Engineering
- Pharmacology
Background:
- Carboxylesterases (CE) play a role in inflammation and drug metabolism.
- Estradiol valerate (EV) is used for inflammation alleviation.
- Monitoring CE levels is crucial for effective and safe hormone therapy.
Purpose of the Study:
- To develop a fluorescent probe (PIPM-CE) for detecting carboxylesterases (CE).
- To utilize PIPM-CE for imaging inflammation alleviation in EV-treated subjects.
- To establish a method for precise monitoring of hormone therapies.
Main Methods:
- Development of a novel fluorescent probe, PIPM-CE.
- Excitation at 368 nm and emission detection at 479 nm for fluorescence signal.
- Establishing correlation between CE levels and fluorescence intensity.
- In vivo imaging of EV-treated inflammation alleviation.
Main Results:
- PIPM-CE demonstrated rapid recognition and a stable reporting signal.
- The probe exhibited high sensitivity, specificity, and low cytotoxicity.
- Successful imaging of EV-treated inflammation alleviation was achieved.
- Correlation between CE levels and fluorescence intensity was established.
Conclusions:
- PIPM-CE is a valuable tool for sensitive and specific CE detection.
- The probe facilitates precise monitoring of inflammation alleviation during EV therapy.
- This work supports optimized hormone therapy and avoidance of overdose-related issues.
