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Detection of geosmin using an aptamer-functionalized LSPR chip integrated with a microfluidic platform
Vivek Semwal1, Ole Bang2, Jakob Janting2
1Manipal Institute of Applied Physics, Manipal Academy of Higher Education, Karnataka, 576104, India; DTU Electro, Department of Electrical and Photonics Engineering, Technical University of Denmark, Kongens Lyngby, 2800, Denmark.
This study introduces a localized surface plasmon resonance (LSPR) sensor for detecting geosmin. The aptamer-functionalized gold nanoparticle sensor achieved a low limit of detection, showing high selectivity for geosmin.
Area of Science:
- Nanotechnology
- Biosensing
- Analytical Chemistry
Background:
- Geosmin is a key odorant compound responsible for earthy off-flavors in water.
- Accurate and rapid detection of geosmin is crucial for water quality monitoring.
- Existing detection methods may lack sensitivity or specificity.
Purpose of the Study:
- To develop a localized surface plasmon resonance (LSPR)-based sensor for sensitive and selective geosmin detection.
- To functionalize gold nanoparticles (AuNPs) with geosmin-specific aptamers for enhanced binding.
- To integrate the aptamer-functionalized LSPR chip into a microfluidic system for reliable measurements.
Main Methods:
- Fabrication of an LSPR chip using 80 nm gold nanoparticles (AuNPs).
- Surface functionalization of the LSPR chip with geosmin-specific aptamers.
- Integration into a microfluidic chamber for real-time binding studies.
- Characterization of bulk refractive index sensitivity (85 nm/RIU).
Main Results:
- The developed LSPR sensor demonstrated a limit of detection (LOD) of approximately 0.6 mg/L for geosmin.
- The sensor exhibited high selectivity, distinguishing geosmin from potential interfering compounds.
- Real-time binding studies confirmed sensor response to geosmin concentrations of 1, 10, and 50 mg/L.
Conclusions:
- The proposed LSPR platform offers a promising approach for rapid and selective geosmin detection.
- The sensor's performance highlights its potential for water quality assessment.
- Incorporating a pre-concentration step could further enhance sensitivity for practical applications.
