Caffeinated Coli: Inspiring the Next Generation of Scientists through Synthetic Biology

Area of Science:

• Synthetic Biology
• Genetic Engineering
• Microbiology Education

Background:

• Inquiry-driven learning enhances STEM education.
• Synthetic biology offers novel applications in research and education.
• Engaging high school students in authentic scientific research is crucial.

Purpose of the Study:

• To introduce high school students to synthetic biology and genetic engineering concepts.
• To provide hands-on experience with bioassay experiments using genetically engineered bacteria.
• To develop a flexible educational module aligned with Next Generation Science Standards (NGSS).

Main Methods:

• Students utilize a genetically engineered *E. coli* strain that grows exclusively on caffeine.
• Two bioassay experiments are conducted, involving bacterial culture creation and growth measurement.
• Caffeine concentrations in unknown samples are calculated using collected experimental data.

Main Results:

• Students successfully created bacterial cultures and measured growth.
• Students calculated caffeine concentrations in unknown samples.
• Positive feedback highlighted collaboration, hands-on learning, and mentor interaction.

Conclusions:

• The Caffeinated Coli Educational Module effectively integrates synthetic biology into high school curricula.
• The module promotes STEM learning and introduces students to 21st-century biology research.
• The program has been successfully implemented in multiple high schools, demonstrating its adaptability and impact.