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Updated: Aug 5, 2026

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
Published on: May 21, 2020
ColonyInsight automated workflow reveals complex dominance in mixed yeast colonies
Tünde Gaizer1, Bence T Gaizer1, Bíborka Pillér1
1Pázmány Péter Catholic University, Faculty of Information Technology and Bionics, Budapest, Hungary.
None:
We present ColonyInsight, a scalable workflow for quantifying microbial interactions in solid phase cultures. Using four Saccharomyces cerevisiae strains tagged with fluorescent markers, we combined time lapse imaging with growth curve analysis to characterize competitive dynamics under different environmental conditions. ColonyInsight integrates a cost effective imaging platform with a dynamic time warping based metric to compare colony growth and infer dominance relationships. Validation by fluorescence microscopy showed that growth based measures reliably estimate competitive outcomes. Applying this approach revealed environment dependent dominance hierarchies across strains in rich and minimal media. ColonyInsight enables systematic, high content analysis of microbial interactions and provides a flexible framework for studying dominance and community behavior across diverse microbial systems.
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