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Updated: Aug 5, 2026

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
An epithelial GPR35 isoform supports tumor-associated transcriptional and metabolic phenotypes
Jørgen D Rønneberg1,2,3, Martine Hjeltnes1,2, Josephine Krieger1,2
1Division of Surgery and Specialized Medicine, Norwegian PSC Research Center, Oslo University Hospital Rikshospitalet, Norway.
Abstract:
G protein-coupled receptor 35 (GPR35) has been implicated in cancer, but the functional roles of its isoforms remain unresolved. Here, we characterize GPR35-long, an N-terminally extended epithelial isoform selectively enriched in colorectal cancer and cholangiocarcinoma. Mass spectrometry and immunohistochemistry confirmed GPR35-long protein expression in tumor cells. Functional analyses revealed that GPR35-long supports tumor-associated transcriptional programs, enhances cellular ATP production, and exhibits increased constitutive and ligand-induced beta-arrestin signaling. These phenotypes were selectively suppressed by the inverse agonist CID-2745687, identifying GPR35-long as a functionally distinct isoform with selective pharmacological sensitivity in tumor cells.
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