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Native Chemical Ligation to Install Chemical Protein Modifications

Eldon R Hard1, Afraah Javed1, Matthew R Pratt2

  • 1Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 1, 2026
PubMed
Abstract

Keywords:
Diselenide-selenoester ligation (DSL)Expressed protein ligation (EPL)Expressed protein selenoester ligation (EPSL)Maleimide protection/deprotectionNative chemical ligation (NCL)Post-translational modifications (PTMs)Protein semisynthesis

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