Related Experiment Video
Updated: Aug 5, 2026

Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection
Published on: May 10, 2024
Pancreas-specific lipid nanoparticle-mediated interleukin-10 mRNA delivery ameliorates acute pancreatitis
Yuqin Men1, Zhi Cao1, David O Popoola1
1Department of Pharmacology, State University of New York, Upstate Medical University, Syracuse, NY 13210, USA. meny@upstate.edu.
Abstract:
Acute pancreatitis (AP) is one of the leading gastrointestinal conditions requiring hospitalization. Current management relies mainly on supportive care, and no specific pharmacological treatment is available. Interleukin-10 (IL-10) is a well-studied cytokine with potent anti-inflammatory and immunomodulatory effects. Previous studies have demonstrated the beneficial roles of both endogenous and exogenous IL-10 in mitigating pancreatic and systemic injury during AP. However, clinical translation of IL-10 recombinant protein therapy has been limited. Here, we report the development of a sulfonium lipid nanoparticle (sLNP) system for pancreas-specific IL-10 mRNA delivery in AP. Through formulation optimization, we identified DHSEA-based sLNPs with excellent pancreas-specific mRNA delivery efficiency, high specificity, and good biocompatibility. Administration of IL-10 mRNA/DHSEA during the development and progression of cerulein-induced AP significantly reduced pro-inflammatory cytokine expression, serum amylase levels, and pancreatic tissue damage.
Related Concept Videos
Acute Pancreatitis II: Clinical Manifestations and Management
Chronic Pancreatitis II: Collaborative Care
Assessment:
Acute Pancreatitis II: Pathophysiology
Chronic Pancreatitis I: Introduction
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
Chronic Pancreatitis I: Introduction
Acute Pancreatitis I: Introduction
