Related Experiment Video
Updated: Aug 5, 2026
Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma
Published on: April 15, 2022
[Correlation of CD269 Expression Patterns with Antigen Expression and Molecular Cytogenetics Abnormalities in
Xian-Feng Wang1,2, Meng Shao1,2, Chao Liang1,2
1Dian Diagnostics Beijing Hematology Diagnostic Center, Beijing 102629, China.
Objective:
To explore the correlation between CD269 expression patterns in multiple myeloma (MM) cells and the expression of other antigens and molecular cytogenetics.
Methods:
Flow cytometry was used to detect the expression level of CD269 in bone marrow plasma cells of 115 newly diagnosed MM patients. The patients were subsequently divided into CD269- group (<20%), CD269 partial expression group (20%-80%), and CD269 high expression group (>80%). The CD269 partial expression group and high expression group were combined as the CD269+ group. The immunophenotype of bone marrow plasma cells was analyzed by flow cytometry. Molecular cytogenetic analysis was performed using combined probe fluorescence in situ hybridization technology.
Results:
There were 27 patients (23.5%) in the CD269- group, 42 patients (36.5%) in the CD269 partial expression group, and 46 patients (40.0%) in the CD269 high expression group. The expression rate of CD56 antigen was 72.7% in the CD269+ group, which was higher than 48.1% in the CD269- group (P <0.05). The expression rates of CD56 and CD117 antigen in the CD269 high expression group were 76.1% and 47.8%, respectively, which were higher than 69.0% and 16.7% in the CD269 partial expression group (both P <0.05). The CD56/CD117 co-expression rate in the CD269 high expression group was 39.1%, which was higher than 14.8% in the CD269- group and 14.3% in the CD269 partial expression group (both P <0.05). The positive rate of IgH/CCND1 gene fusion in the CD269- group was 63.0%, which was higher than 31.0% in the CD269 partial expression group, 19.6% in the CD269 high expression group, and 25.0% in the CD269+ group (all P <0.05). The IgH deletion rate was 13.0% in the CD269 high expression group, while no IgH deletion was observed in the CD269 partial expression group, with a statistically significant difference between the two groups (P <0.05).
Conclusion:
The expression of CD269 on bone marrow plasma cells in MM patients is significantly correlated with the expression of CD56 and CD117, as well as IgH/CCND1 gene fusion and IgH deletion. The high expression of CD269 is associated with better prognosis related biological indicators.
