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Updated: Aug 5, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
[Analysis of Factor Ⅷ and Fibrinogen Levels and Influencing Factors in Cryoprecipitate Initiating Blood]
Dan-Xia Zhang1, Guang Shi1, Peng Liu1
1Qingdao Blood Center, Qingdao 266073, Shandong Province, China.
Objective:
To investigate the levels of factor Ⅷ (FⅧ) and fibrinogen (FIB) in cryoprecipitate initiating blood (fresh frozen plasma, FFP) and explore the influence of ABO blood type, gender, and age on the levels of FⅧ and FIB in FFP among blood donors in Qingdao.
Methods:
A total of 260 bags of cryoprecipitate initiating blood were prepared according to the Technical Operating Procedures for Blood Stations (2019 version). FⅧ and FIB levels were measured, and statistical analyses including t-tests, rank-sum tests, one-way ANOVA with LSD method, and multiple linear regression were performed to analyze the influence of ABO blood type, gender, and age on the levels of FⅧ and FIB.
Results:
The FⅧ level in cryoprecipitate initiating blood of blood donors in Qingdao was found to be (1.08±0.57) IU/mL. The FⅧ levels in A, B, and AB blood type groups were higher than in O blood type group (P<0.05). Female individuals showed higher FⅧ levels compared to males (P<0.05). Moreover, the FⅧ levels in the 31-40 and 41-58 age groups were significantly higher than that of the 18-30 age group (P<0.05). The FIB level in cryoprecipitate initiating blood was (2.18±0.44) g/L. The FIB levels of female group was higher than that of male group (P<0.05). There were statistically significant differences in FIB levels among different age groups (P<0.05). Multiple linear regression analysis showed that blood group and age were independent factors influencing FⅧ content, age was an independent influence of FIB content.
Conclusion:
ABO blood type was identified as an influencing factor for FⅧ levels in cryoprecipitate initiating blood, with the O blood type having the lowest levels. Age was an influencing factor for both FⅧ and FIB levels, with levels increasing with age. Additionally, females individuals showed higher levels of both FⅧ and FIB compared to males.
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