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Updated: Aug 5, 2026
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Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper (Capsicum annuum L.)
Published on: November 30, 2022
Genome-Wide Identification and Functional Characterization of CaGRAS43 in Enhancing Cold Stress Tolerance in Pepper
Wenbin Yuan1, Ikram Ullah2, Rupa Mishra3
1College of Agronomy, Chuxiong Normal University, Chuxiong, China.
Physiologia Plantarum
|August 3, 2026
Summary
Pepper plants possess a GRAS gene, CaGRAS43, that enhances cold tolerance. This gene regulates physiological and molecular responses, offering potential for engineering cold-resilient crops.
Area of Science:
- Plant Biology
- Molecular Genetics
- Agricultural Science
Background:
- Cold stress significantly hinders plant growth and crop yields.
- Identifying genes that confer cold tolerance is crucial for agricultural productivity.
Purpose of the Study:
- To identify and analyze the GRAS transcription factor family in pepper (Capsicum annuum) under cold stress.
- To investigate the role of specific GRAS genes, particularly CaGRAS43, in mediating cold stress responses.
Main Methods:
- Genome-wide identification and in silico analysis of pepper GRAS genes.
- Expression analysis under cold conditions.
- Virus-induced gene silencing (VIGS) and overexpression studies in pepper and Arabidopsis.
Main Results:
- 64 GRAS genes were identified in the pepper genome.
- CaGRAS43 expression increased under cold stress.
- Silencing CaGRAS43 enhanced cold sensitivity, while overexpression improved tolerance by reducing oxidative damage and enhancing defense gene expression.
Conclusions:
- CaGRAS43 acts as a positive regulator of cold stress tolerance in pepper.
- It modulates physiological and molecular mechanisms to enhance plant resilience.
- CaGRAS43 is a promising target for developing cold-resilient pepper and other crops.
