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Published on: June 7, 2024
Phenotypical, physiological and molecular identification in Bougainvillea × buttiana 'Miss Manila' leaves by
Kunlin Wu1, Hongling Lin1, Jiameng Wang1
1Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication)/School of Tropical Agriculture and Forestry, Hainan University, Sanya, 572025, China; Engineering Research Center of Rare and Precious Tree Species in Hainan Province, School of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China.
Abstract:
The beautiful bract coloration of Bougainvillea × buttiana 'Miss Manila' renders it significant for both landscaping and as a key source material for betaine extraction. To understand its response to gamma rays irradiation, we exposed cuttings to varying doses of 60Co-γ radiation and integrated physiological assessments and transcriptome profiling. The results demonstrated that radiation significantly altered the physiological and biochemical characteristics of the leaves. The median lethal dose value is 30-38 Gy, with the highest relative mutation rate observed at a radiation dose of 30 Gy. Antioxidant enzyme activities responded differentially: superoxide dismutase (SOD) activity decreased, while catalase (CAT) activity increased with rising radiation dose. Transcriptome analysis revealed 2211 differentially expressed genes, including 1288 up- and 923 down-regulated, enriched in pathways related to antioxidant defense, osmotic regulation, and DNA repair. qRT-PCR results further confirmed the significant upregulation of antioxidant-related gene IMT1 and DNA repair-related genes CARP1, PBP1, among others. These results not only uncover the physiological and molecular mechanisms underpinning radiation tolerance, but also provide a foundational molecular framework for gamma rays mutation breeding in Bougainvillea.
