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Published on: February 28, 2015
A gold-palladium bimetallic nanozyme-based colorimetric sensor for nitrite assay
Xinyuan Xu1,2, Chenlu Shi1,2, Xiaolin Qu1,2
1University of Science and Technology of China, Hefei 230026, China.
The Analyst
|August 4, 2026
Summary
Gold-palladium (Au-Pd) nanozymes show enhanced peroxidase-like activity for sensitive nitrite detection. This novel colorimetric assay offers a simple, accurate method for food safety and environmental monitoring.
Area of Science:
- Nanomaterials Science
- Analytical Chemistry
- Biochemistry
Background:
- Bimetallic nanozymes offer synergistic advantages over single-metal counterparts, improving stability and catalytic efficiency.
- Gold-palladium (Au-Pd) nanozymes are synthesized to harness these benefits for advanced sensing applications.
Purpose of the Study:
- To synthesize a gold-palladium (Au-Pd) bimetallic nanozyme using a one-step co-reduction method.
- To develop a sensitive colorimetric assay for the quantitative detection of nitrite.
Main Methods:
- Synthesis of Au-Pd bimetallic nanozymes via a one-step co-reduction technique.
- Utilizing the nanozyme's peroxidase-like activity to catalyze TMB oxidation.
- Developing a colorimetric detection strategy based on nitrite's reaction with oxidized TMB.
Main Results:
- The synthesized Au-Pd nanozyme demonstrated high peroxidase-like activity and substrate affinity.
- A colorimetric assay was established for nitrite detection with a linear range of 5–500 μM.
- The assay achieved a low limit of detection (0.23 μM) and exhibited high sensitivity and accuracy.
Conclusions:
- The Au-Pd bimetallic nanozyme provides a robust platform for sensitive nitrite detection.
- The developed colorimetric method is suitable for reliable nitrite quantification in food safety and environmental monitoring.
