Related Experiment Videos
LncRNA-XIST Competitively Binds to MicroRNA-532-5p to Regulate PTEN Expression, Exacerbating Cerebral
Xudong Lu1,2, Luhan Chen1,2, Liqi Qian1,2
1Department of Neurology, Jiaxing Second Hospital, Jiaxing, Zhejiang, China.
Advanced Biology
|August 5, 2026
Summary
Long non-coding RNA-XIST promotes cerebral ischemia-reperfusion injury (CIRI) by targeting microRNA-532-5p and PTEN. Inhibiting lncRNA-XIST may offer a therapeutic strategy for CIRI.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Cerebral ischemia-reperfusion injury (CIRI) is a significant neurological condition with unclear molecular mechanisms.
- Long non-coding RNA-XIST (lncRNA-XIST) has been implicated in CIRI, but its specific role and pathways are not fully understood.
Purpose of the Study:
- To elucidate the mechanism by which lncRNA-XIST contributes to cerebral ischemia-reperfusion injury.
- To investigate the regulatory axis involving lncRNA-XIST, microRNA-532-5p, and PTEN in CIRI.
Main Methods:
- Established middle cerebral artery occlusion/reperfusion (MCAO/R) rat and oxygen-glucose deprivation/reoxygenation (OGD/R) SH-SY5Y cell models.
- Utilized qPCR, bioinformatic prediction, dual-luciferase assays, CCK-8, flow cytometry, Western blot, and MDA/SOD assays to assess molecular changes and cellular responses.
- Investigated the expression levels of lncRNA-XIST, microRNA-532-5p, and PTEN.
Main Results:
- LncRNA-XIST and PTEN expression were elevated, while microRNA-532-5p was reduced in CIRI models.
- Knockdown of lncRNA-XIST mitigated OGD/R-induced cellular injury, apoptosis, and oxidative stress.
- LncRNA-XIST directly targets and inhibits microRNA-532-5p, which in turn targets and suppresses PTEN. This regulatory axis was confirmed through knockdown and rescue experiments.
Conclusions:
- LncRNA-XIST exacerbates cerebral ischemia-reperfusion injury through the microRNA-532-5p/PTEN pathway.
- Targeting the lncRNA-XIST/microRNA-532-5p/PTEN axis presents a potential therapeutic avenue for treating CIRI.
Related Concept Videos
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
03:17Inheritance of Chromatin Structures
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
02:15Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...