Related Experiment Video
Updated: Aug 7, 2026
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
Published on: January 29, 2020
Protocol for non-invasive genotyping of axolotls using skin swabbing
Laura Isabella Arbanas1, Aida Rodrigo Albors1
1Centre for Regenerative Medicine, Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh EH16 4UU, UK; Institute for Stem Cell Research, School of Biological Sciences, The University of Edinburgh, Edinburgh EH16 4UU, UK.
Abstract:
Fin clipping is the standard method for sampling axolotl DNA for genotyping, but it requires anesthesia and removing a piece of tissue. Here, we present a non-invasive protocol for axolotl DNA sampling and genotyping using skin swabbing. We describe the steps for extracting DNA from skin cells collected with cotton swabs and then detail how to sex genotype axolotls using PCR and gel electrophoresis. This protocol removes the need to cause an injury before regeneration experiments and improves axolotl welfare.
