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Extraction process, structural features, and bioactivity validation of Dendrobium officinale polysaccharides
1Wuhu Institute of Technology, Wuhu, PR China.
Carbohydrate Research
|August 5, 2026
Summary
An optimized process efficiently extracts Dendrobium officinale polysaccharides (DOPs). Two fractions, DOP-1 and DOP-2, show distinct structures and protect skin cells from photoaging by regulating the TGF-β/Smad pathway.
Area of Science:
- Biochemistry
- Pharmacology
- Cell Biology
Background:
- Dendrobium officinale polysaccharides (DOPs) are known for their biological activities.
- Optimizing extraction and purification is crucial for obtaining homogeneous fractions with specific properties.
- Understanding the structural characteristics of DOPs is key to elucidating their functional mechanisms.
Purpose of the Study:
- To optimize the extraction-purification process for Dendrobium officinale polysaccharides (DOPs).
- To characterize the fine structures of two homogeneous fractions, DOP-1 and DOP-2.
- To evaluate the anti-photoaging activity of DOP-1 and DOP-2 on human foreskin fibroblast (HFF) cells.
Main Methods:
- An integrated extraction-purification process involving defatting, enzymatic hydrolysis, ethanol precipitation, deproteinization, decolorization, and dialysis was employed.
- DEAE-52 and Sephadex G-100 chromatography were used to separate and purify DOP-1 and DOP-2.
- Structural characterization utilized FT-IR, methylation-GC/MS, NMR, and SEM. Anti-photoaging effects were assessed using CCK-8 assays, SA-β-Gal staining, and qPCR analysis of the TGF-β/Smad pathway.
Main Results:
- The optimized process significantly increased crude polysaccharide yield and content.
- DOP-1 was identified as a linear oligosaccharide, while DOP-2 was a branched polysaccharide with distinct monosaccharide compositions and linkages.
- Both DOP-1 and DOP-2 demonstrated protective effects against UVA-induced photoaging in HFF cells, modulating key genes in the TGF-β/Smad signaling pathway, including upregulation of TGF-β1, Smad3, Smad4, and Collagen I, and downregulation of Smad7 and MMP-1. DOP-2 exhibited dose-dependent effects.
Conclusions:
- The optimized process provides an efficient method for preparing DOPs.
- DOP-1 and DOP-2 possess unique structural features that contribute to their anti-photoaging properties.
- The anti-photoaging effects are mediated through the regulation of the TGF-β/Smad signaling pathway.
