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Diagnostic value of miR-34c-5p in acute cerebral infarction and its correlation with prognostic outcomes of
Hongjie Jiang1, Xin Zhang2, Wei Wang1
1Rehabilitation Department 3, Beidahuang Group General Hospital, Heilongjiang, China.
Objective:
This study aimed to explore the role of miR-34c-5p in ACI to provide novel insights for ACI clinical management.
Methods:
This study enrolled 376 subjects (176 healthy individuals, 200 ACI patients). Serum miR-34c-5p expression was quantified by qRT-PCR, with its diagnostic potential evaluated by receiver operator characteristic (ROC) curve. The association between miR-34c-5p and ACI severity was evaluated by correlation analysis. Dual-luciferase assay validated miR-34c-5p/FOSL1 interaction. An oxygen-glucose deprivation and reperfusion (OGD/R)-induced SH-SY5Y model was employed for evaluating the effect of miR-34c-5p/FOSL1 axis on cerebral ischemia-reperfusion injury (CIRI).
Results:
MiR-34c-5p downregulation possessed a diagnostic potential in ACI. The miR-34c-5p expression, negatively correlating with the NIHSS scores of ACI subjects, was lower in ACI subjects with moderate/severe severity and unfavorable prognosis. MiR-34c-5p downregulation and higher NIHSS scores were risk factors for ACI poor prognosis. OGD/R reduced cell viability, facilitated inflammation (IL-6, TNF-α), and oxidative stress (decreased SOD activity, increased MDA level) in SH-SY5Y cells. MiR-34c-5p overexpression protected against OGD/R-induced SH-SY5Y injury by improving viability, suppressing inflammation, and oxidative stress via targeting FOSL1.
Conclusions:
Downregulation of miR-34c-5p showed a diagnostic potential on ACI and was correlated with ACI severity and prognosis.