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Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
Published on: November 29, 2024
NeuroSuite for Long-Term Functional and Structural Studies of Air-Liquid Interface Cerebral Organoids
Belquis Haider1, Sagnik Middya1, Daniel J Lloyd-Davies-Sánchez2
1Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|August 6, 2026
Summary
NeuroSuite enables long-term neural monitoring of human cerebral organoids (ALI-COs) using a novel microelectrode array. This platform facilitates detailed study of brain development and disease models over months.
Area of Science:
- Neuroscience
- Biotechnology
- Developmental Biology
Background:
- Long-term electrophysiological monitoring of cerebral organoids is limited by tissue necrosis.
- Existing methods struggle with stable, in situ recordings over extended periods.
Purpose of the Study:
- To introduce NeuroSuite, a modular platform for sustained, spatially resolved neural monitoring of air-liquid interface cerebral organoids (ALI-COs).
- To enable detailed analysis of organoid network dynamics and maturation over months.
Main Methods:
- Development of Neuroweb, an ultrathin, perforated microelectrode array for ALI-COs.
- Utilizing NeuroMaps, an open-source analysis suite for signal processing and data integration.
- Validation through immunofluorescence, RNA sequencing, and ex vivo rat brain slice recordings.
Main Results:
- Neuroweb preserves cellular diversity and enables low-impedance recordings.
- NeuroSuite supports stable recordings beyond 180 days.
- Demonstrated capture of maturation-dependent network dynamics and oscillatory activity.
Conclusions:
- NeuroSuite provides a robust platform for long-term neural monitoring of ALI-COs.
- The system is versatile for developmental neuroscience, disease modeling, and drug screening.
- Facilitates in-depth study of human brain development and function in vitro.
