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Updated: Aug 7, 2026
08:19
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
Published on: July 7, 2020
[Carbapenemase-producing Enterobacterales]
Barbara Juliane Holzknecht1,2,3, Christian Salgård Jensen4, Anne Line Engsbro5
1Afdeling for Klinisk Mikrobiologi, Københavns Universitetshospital - Herlev og Gentofte Hospital.
Ugeskrift for Laeger
|August 6, 2026
Summary
Carbapenemase-producing Enterobacterales (CPE) cases in Denmark have surged tenfold due to healthcare-associated outbreaks. Updated screening and specialist-guided treatment are crucial as resistance to new agents emerges.
Area of Science:
- Infectious Diseases
- Microbiology
- Public Health
Background:
- Carbapenemase-producing Enterobacterales (CPE) pose a significant global and national health threat.
- Denmark has experienced a tenfold increase in CPE cases over the last decade.
- Nosocomial outbreaks are a primary driver, with some involving up to 100 patients over extended periods.
Purpose of the Study:
- To review the epidemiology of CPE in Denmark.
- To assess the adequacy of current screening protocols.
- To discuss challenges in CPE treatment and emerging resistance.
Main Methods:
- Review of epidemiological data on CPE in Denmark.
- Analysis of current screening strategies for CPE.
- Discussion of treatment guidelines and antimicrobial resistance patterns.
Main Results:
- A significant 10-fold increase in diagnosed CPE cases in Denmark over ten years.
- Identification of nosocomial outbreaks as a major contributing factor.
- Emergence of acquired resistance to newly approved antimicrobial agents.
Conclusions:
- Current screening algorithms for CPE require revision to reflect evolving epidemiology.
- CPE management necessitates specialized, expert-guided treatment approaches.
- Antimicrobial resistance to new agents is a growing concern, complicating treatment options.
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