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Published on: January 17, 2017
Model-Assisted Prioritization of Gelatin Methacryloyl (GelMA) Hydrogel Formulations for Three-Dimensional Cell
Peisong Yin1,2, Xin Wang2, Suqing Liu2
1Biomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou510515, China.
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Owing to highly tunable mechanics, gelatin methacryloyl (GelMA) hydrogels are widely exploited for three-dimensional (3D) cell culture, whereas limited experimental sampling restricts efficient formulation screening. In this work, we developed a BNN-based modeling pipeline to map GelMA hydrogels with various cross-linking parameters toward linear viscoelastic moduli and nonlinear critical stress, thereby categorizing all tested formulations into low/intermediate/high stable mechanical windows. Calibration on C2C12 myoblast morphologies confirmed that nonlinear critical stress complements linear rheological parameters to refine the screening priority of cell-compatible hydrogel recipes. Subsequent validation with primary cardiomyocytes demonstrated consistent morphological trends matching the predefined mechanical windows, alongside ambiguous boundary formulations. Our findings construct a bounded prioritization strategy to rapidly select GelMA compositions under sparse experimental conditions, with further prospective validations demanded before generalized predictive use for diverse tissue engineering scenarios.
