An osmium-modified Prussian blue heterostructure for biofilm disruption and oxidative stress to combat
Xia Chen1, Xinlin Jia2, Chaohong Yu2
1Lab of Low-Dimensional Materials Chemistry, Key Laboratory for Ultrafine Materials of Ministry of Education, Frontier Science Center of the Materials Biology and Dynamic Chemistry, Shanghai Engineering Research Center of Hierarchical Nanomaterials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
Abstract:
One major contributor to the therapeutic recalcitrance of methicillin-resistant Staphylococcus aureus (MRSA) infections is its robust biofilm formation, which impedes therapeutics penetration, diminishes bacterial susceptibility to exogenous stimulus and exacerbates host inflammation, collectively leading to suboptimal therapeutic outcomes. To address these interrelated challenges, we developed an osmium-modified cobalt‑iron Prussian blue analogue (OsCoPBA) heterostructure with high-affinity binding to MRSA biofilm and exceptional photothermal conversion efficiency under near-infrared (NIR) irradiation, thereby enabling effective biofilm disruption. The OsCoPBA heterostructure was characterized by its redox property, imparting both pro-oxidant and anti-oxidant capabilities under different conditions. In acidic wounds, OsCoPBA exhibited a peroxidase (POD)-like activity, generating bactericidal reactive oxygen species (ROS) to induce oxidative stress within MRSA. Conversely, during the wound healing phase, OsCoPBA exhibited robust superoxide dismutase (SOD)-like activity (a peak ·O₂- scavenging efficiency of 95% at the optimal concentration of 250 μg mL-1) and enhanced catalase (CAT)-like activity, effectively scavenging excess ROS to suppress inflammation. This heterostructure achieved on-demand regulation of ROS for meeting the antibacterial and anti-inflammatory requirements, representing a rational design strategy for managing MRSA-infected wounds.
Insights
A novel osmium-modified cobalt-iron Prussian blue analogue (OsCoPBA) effectively disrupts methicillin-resistant Staphylococcus aureus (MRSA) biofilms. This material offers on-demand reactive oxygen species (ROS) regulation for antibacterial and anti-inflammatory effects in wound healing.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Infectious Diseases
Background:
- Methicillin-resistant Staphylococcus aureus (MRSA) infections are challenging due to robust biofilm formation.
- Biofilms impede drug penetration, reduce bacterial susceptibility, and worsen inflammation, leading to poor treatment outcomes.
Purpose of the Study:
- To develop a novel heterostructure for effective MRSA biofilm disruption and wound management.
- To engineer a material with tunable redox properties for both antibacterial and anti-inflammatory actions.
Main Methods:
- Synthesis of an osmium-modified cobalt-iron Prussian blue analogue (OsCoPBA) heterostructure.
- Characterization of OsCoPBA's redox properties, photothermal conversion efficiency, and binding affinity to MRSA biofilms.
- Evaluation of OsCoPBA's peroxidase-like (pro-oxidant) and superoxide dismutase/catalase-like (antioxidant) activities under different conditions.
Main Results:
- OsCoPBA demonstrated high-affinity binding to MRSA biofilms and efficient photothermal conversion under near-infrared irradiation.
- In acidic conditions, OsCoPBA exhibited peroxidase-like activity, generating reactive oxygen species (ROS) to kill MRSA.
- During wound healing, OsCoPBA showed significant superoxide dismutase-like and catalase-like activities, scavenging excess ROS to reduce inflammation.
Conclusions:
- The OsCoPBA heterostructure provides on-demand regulation of ROS, addressing both antibacterial and anti-inflammatory needs in MRSA-infected wounds.
- This rational design strategy offers a promising approach for managing challenging MRSA wound infections.
