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Updated: Aug 8, 2026
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
Photoactivated liquid-liquid phase separation-based cascade CRISPR/Cas12a electrochemical platform for sensitive
Yiping Zhu1, Shihua Luo2, Min Luo3
1Guangzhou University of Chinese Medicine Science and Technology Innovation Center, 12 Airport Road, Baiyun District, Guangzhou, Guangdong, 510405, PR China.
This study introduces a novel electrochemical platform for detecting small extracellular vesicles (sEVs) surface proteins, crucial for early gastric cancer (GC) diagnosis. The method offers high sensitivity and specificity, enabling accurate discrimination between GC patients and healthy individuals.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Oncology
Background:
- Small extracellular vesicles (sEVs) are promising biomarkers for gastric cancer (GC) liquid biopsy.
- Current methods for profiling sEV surface proteins face challenges in sensitivity and background interference in clinical samples.
Purpose of the Study:
- To develop an ultrasensitive and specific electrochemical platform for detecting sEV surface proteins.
- To enable precise spatiotemporal control over the detection reaction for improved efficiency and reduced background.
Main Methods:
- Development of a photoactivated liquid-liquid phase separation cascaded Cas12a amplification (PL-cCas12a) electrochemical platform.
- Utilizing aptamers targeting GC-associated sEV surface proteins for specific detection.
- Leveraging photoactivation for precise reaction initiation and a confined microenvironment for enhanced efficiency.
Main Results:
- Achieved a limit of detection (LOD) as low as 3.95 × 10³ particles/μL with minimal background leakage.
- The PL-cCas12a platform demonstrated high accuracy in discriminating GC patients from healthy donors (AUC = 0.912).
- The system exhibited precise spatiotemporal control, accelerated reaction efficiency, and suppressed non-specific trans-cleavage.
Conclusions:
- The developed PL-cCas12a electrochemical platform offers ultrasensitive and specific detection of sEV surface proteins.
- This platform shows significant potential as a tool for non-invasive early diagnosis of gastric cancer.
- The precise control and high performance address limitations of existing methods for sEV profiling.
