DTaP-IPV Vaccination-Induced Granuloma Diagnosed With Multi-Virus/Microbial Real-Time PCR

Vaccination-induced granuloma is a common clinical diagnosis, but few reports have described the diagnostic utility of comprehensive pathological and microbiological examinations. This report details a case of DTaP-IPV vaccination-induced granuloma where the use of multi-virus/microbial real-time polymerase chain reaction (PCR) enabled a definitive molecular diagnosis. This advanced testing method confirmed the presence in the excised tissue of Bordetella pertussis and poliovirus genes from the vaccine components, a novel finding for this type of lesion. In cases requiring a definitive diagnosis, especially those where malignancy is a differential, surgical excision followed by multiplex PCR testing is a reliable option.