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Updated: Aug 8, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
Published on: December 9, 2022
Enzymatic Bioautographic Methods
I Ayelen Ramallo1, Mario O Salazar1, Ricardo L E Furlan2
1Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario-CONICET, Rosario, Argentina.
Abstract:
Enzymatic bioautography enables the detection of enzyme inhibitors absorbed on a thin-layer chromatography plate, making it particularly useful for identifying inhibitors in complex mixtures. This technique allows the direct analysis of the inhibitory properties of compounds separated by thin-layer chromatography, generating an inhibition profile. Here, we describe the conditions to detect inhibitors of the xanthine oxidase and β-glucosidase immobilized on agar gel.
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